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02004-500 AccuRuler 1Kb Plus DNA Ladder

02004-500 AccuRuler

1Kb Plus DNA Ladder

Description

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Size: 50μg / 500μl

MaestroGen DNA Ladders, comprising of fragments of specific base pair length, are available for estimation of molecular weights from 100 bp to 10 Kb. For easy determination of band size, several DNA Ladders include bands that are two to three times more intense. 

02004-500 AccuRuler 1 Kb Plus DNA ready-to-use Ladder is suitable for sizing linear double stranded DNA fragments from 100 bp to 10 Kb. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The 1 Kb and 3 Kb bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (assuming a 0.5 μg / 5 μl load) for approximating the mass of DNA in comparably intense samples of similar size.

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MaestroGen DNA Ladder 02001-500, 02002-500, 02004-500 (358 KB)
Features

Accuracy
Accurate size and mass estimations 

Bands Increased Intensity
1 Kb and 3 Kb bands have increaesd intensity

RTU
Ready to use

Source
PCR products and double-stranded DNA digested with appropriate restriction
enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0)
and 10mM EDTA

Specification
Cat. No.   02004-500
Size   50 μg / 500 μl
Range   100 bp to 10 Kb
Bands Increased Intensity   1 Kb and 3 Kb bands
Number of Bands   13
Concentration   114 μg / ml
Recommended Load   5 μl per well
Mass/Band   0.5 μg / 5 μl
Storage & Loading Buffer
 
  10 mM Tris-HCl (pH 8), 10 mM EDTA, bromophenol blue, xylene cyanol
FF and glycerol
Storage

 
  24 months at -20°C
12 months at 4°C
6 months at 25°C

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