DNA Stain, Loading Dye
MR-031203 MaestroSafe Nucleic Acid Prestained Dye

MR-031203 MaestroSafe

Nucleic Acid Prestained Dye


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*Only available on UV lamp, DNA ladder, protein ladder and DNA stain (loading dye). 

Size: 1 ml

MaestroSafeTM Nucleic Acid Prestained Dye represent a new and safe class of nucleic acid dye for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dye is developed to replace toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels.

MaestroSafeTM Nucleic Acid Prestained Dye is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

With MaestroSafeTM Nucleic Acid Prestained Dye, you do not need to do post-stain or pre-mix with agarose gel. All you have to do is mix the dye with agarose in process of preparation and run electrophoresis normally. After the electrophoresis, view and document your results as you do with EtBr staining protocols.

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Download:MaestroSafe_MR-031201&MR-031203_Catalog (1.8 MB)
Download:MaestroGen_MR-031203_MSDS (108 KB)
The University of Edinburgh in UK recommends MaestroSafe TM : The University of Edinburgh

MaestroSafe TM Nucleic Acid Loading Dye emits green fluorescence for dsDNA or ssDNA, and red fluorescence for RNA.This dye has one excitation (470 nm) and two emission spectra (520 nm and 635 nm).

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Cloning efficiency of MaestroSafe TM and LED light, compared with EtBr and UV light:
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According to the cloning efficiency test, DNA stained with EtBr and exposed by UV light will reduce the efficiency of cloning. The combination of MaestroSafe TM and LED light shows a better cloning efficiency.

Cat. No.   MR-031203
Size    1ml
Description   Prestained with agarose
Storage   12 months at 4°C
Protocol   For laboratory only. Not for clinical applications.
    1. Prepare a 100ml agarose solution.
    2. Heat solution until particles disappeared.
    3. Add 1.5-2μl MaestroSafeTM Nucleic Acid Prestained Dye into solution and mix gently.
    4. Let the solution cool down to 60-70℃ and cast the gel.
    5. Load samples into well and perform electrophoresis.
    6. View the results under traditional UV light after electrophoresis.