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MR-031203 MaestroSafe Nucleic Acid Prestained Dye

MR-031203 MaestroSafe

Nucleic Acid Prestained Dye

Description

News!
The University of Edinburgh in UK recommends MaestroSafeTM:
Webpage: The University of Edinburgh

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Size: 1 ml

MaestroSafeTM Nucleic Acid Prestained Dye represent a new and safe class of nucleic acid dye for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dye is developed to replace toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels.

MaestroSafeTM Nucleic Acid Prestained Dye is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

With MaestroSafeTM Nucleic Acid Prestained Dye, you do not need to do post-stain or pre-mix with agarose gel. All you have to do is mix the dye with agarose in process of preparation and run electrophoresis normally. After the electrophoresis, view and document your results as you do with EtBr staining protocols.

View More Details
Download: MaestroSafe MR-031201 MR-031203 Catalog (1.8 MB)
Features
MaestroSafeTM Nucleic Acid Prestained Dye emits green fluorescence when bound to dsDNA or ssDNA, and red fluorescence when bound to RNA. This dye has one excitation (470 nm) and two emission spectra (520 nm and 635 nm).     
MaestroSafeTM Nucleic Acid Prestained Dye is compatible with MaestroGen LED Transilluminator or Gel Documentation System: 
Product LED Transilluminator Gel Documentation System
Product UltraSlim UltraBright Mini-Imager MiniCute Imager
Photo

Cat No.

SLB-01W
SLB-01

LB-16 MI-01 MC-02
Viewing size
(cm)
7 x 12 16 x 20 8 x 15 7 x 12 or 16 x 20

Wavelength
(nm)

470 470 302 & 470 470


Cloning efficiency of MaestroSafeTM and LED light, compared with EtBr and UV light:

According to the cloning efficiency test, DNA stained with EtBr and exposed by UV light will reduce the efficiency of cloning. The combination of MaestroSafeTM and LED light shows the better cloning efficiency. It demonstrates that MaestroSafeTM and LED light will enhance cloning efficiency.
Specification
Size    1ml
Description   Prestained with agarose
Protocol   For laboratory only. Not for clinical applications.
    1. Prepare a 100ml agarose solution.
    2. Heat solution until particles disappeared.
    3. Add 1.5-2μl MaestroSafeTM Nucleic Acid Prestained Dye into solution and mix gently.
    4. Let the solution cool down to 60-70℃ and cast the gel.
    5. Load samples into well and perform electrophoresis.
    6. View the results under traditional UV light after electrophoresis.
    7. Or use MaestroGen UltraSlim or UltraBright LED Transilluminator for not only good performance
    but health concerns.
History
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