Size: 50μg / 500μl
The AccuRuler 100 bp Plus DNA RTU Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 3,000 bp.
The 500 bp and 1,500 bp bands have increased intensity to provide internal orientation.
The mass of DNA in each band is provided (assuming a 0.5 μg/ 5 μl load) for approximate
quantification in comparably intense samples of similar size.
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Source PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1mM EDTA.
Range: 100 bp to 3,000 bp
Number of bands: 12 Concentration: 100 μg/ml
Recommended load: 5 μl per well
Storage and loading buffer: 10 mM Tris-HCl (pH 8), 10 mM EDTA, orange G, xylene cyanol FF and glycerol.
Storage: Store at 25°C for 6 months and at -20°C for 24 months