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DNA Ladder
02002-500 AccuRuler 100bp Plus DNA Ladder

02002-500 AccuRuler

100bp Plus DNA Ladder

Description

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*Only available on UV lamp, DNA ladder, protein ladder and DNA stain (loading dye). 

Size: 50μg / 500μl

MaestroGen DNA Ladders, comprising of fragments of specific base pair length, are available for estimation of molecular weights from 100 bp to 10 Kb. For easy determination of band size, several DNA Ladders include bands that are two to three times more intense. 

02002-500 AccuRuler 100 bp Plus DNA ready-to-use Ladder is suitable for sizing linear double stranded DNA fragments from 100 bp to 3,000 bp. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The 500 bp and 1,500 bp bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (assuming a 0.5 μg / 5 μl load) for approximating the mass of DNA in comparably intense samples of similar size.

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MaestroGen DNA Ladder 02001-500, 02002-500, 02004-500 (358 KB)
Features

Accuracy
Accurate size and mass estimations 

Bands Increased Intensity
500 bp and 1,500 bp bands have increaesd intensity

RTU
Ready to use

Source
PCR products and double-stranded DNA digested with appropriate restriction
enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0)
and 10mM EDTA

Specification
Cat. No.   02002-500
Size   50 μg / 500 μl
Range   100 bp to 3,000 Kb
Bands Increased Intensity   500 bp and 1,500 bp bands
Number of Bands   12
Concentration   100 μg / ml
Recommended Load   5 μl per well
Mass/Band   0.5 μg / 5 μl
Storage & Loading Buffer
 
  10 mM Tris-HCl (pH 8), 10 mM EDTA, orange G, xylene cyanol FF
and glycerol
Storage

 
  24 months at -20°C
12 months at 4°C
6 months at 25°C
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