Size: 50μg / 500μl
The AccuRuler 1 kb DNA RTU Ladder is suitable for sizing linear doublestranded DNA
fragments from 250 bp to 10 kb.
The 1 kb and 3kb bands have increased intensity to provide internal orientation.
The mass of DNA in each band is provided (assuming a 0.5 μg/ 5 μl load) for approximate
quantification in comparably intense samples of similar size.
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Source PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1mM EDTA.
Range: 250 bp to 10 kb
Number of bands: 13
Concentration: 100 μg/ml
Recommended load: 5 μl per well
Storage and loading buffer: 10 mM Tris-HCl (pH 8), 10 mM EDTA, bromophenol blue and glycerol.
Storage: Store at 25°C for 6 months and at -20°C for 24 months.